A Genetic Manipulation of Motor Neuron Excitability Does Not Alter Locomotor Output in Drosophila Larvae

1 Are motor neurons just passive relayers of the signals they receive? Or, do motor 2 neurons shape the signals before passing them on to the muscles, thereby influencing 3 the timing of motor behavior? Few direct tests of the role of motor neuron intrinsic 4 properties in shaping motor behavior have been carried out, and many questions remain 5 about the role of specific ion channel genes in motor neuron function. In this study, two 6 potassium channel transgenes were expressed in Drosophila larval motor neurons to 7 increase their excitability. Mosaic animals were created in which some identified motor 8 neurons expressed the transgenes while others did not. Motor output underlying 9 crawling was compared in muscles innervated by control and experimental neurons in 10 the same animals. No effect of the transgenic manipulation on motor output was seen. 11 Possible explanations for these results are discussed, and future experiments are 12 outlined that could shed light on how the larval nervous system produces normal motor 13 output in the face of altered motor neuron excitability. 14